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Last updated: 2020-12-28
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| File | Version | Author | Date | Message |
|---|---|---|---|---|
| html | 0a845cc | Sean Wilson | 2020-12-28 | update to flatly theme |
| html | 07adb2a | Sean Wilson | 2020-12-28 | Build site. |
| Rmd | 11477de | Sean Wilson | 2020-12-28 | Updating additional information around analysis |
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| html | 84370d5 | Sean Wilson | 2020-11-13 | Build site. |
| Rmd | 96a2303 | Sean Wilson | 2020-11-09 | Start workflowr project. |
Howden, Wilson et al. 2020
This repository is a workflowr project showing the analysis and output relating to Howden, Wilson et al. 2020: Plasticity of distal nephron epithelia from human kidney organoids enables the induction of ureteric tip and stalk; Cell Stem Cell
Graphical Abstract
Summary
During development, distinct progenitors contribute to the nephrons versus the ureteric epithelium of the kidney. Indeed, previous human pluripotent stem-cell-derived models of kidney tissue either contain nephrons or pattern specifically to the ureteric epithelium. By re-analyzing the transcriptional distinction between distal nephron and ureteric epithelium in human fetal kidney, we show here that, while existing nephron-containing kidney organoids contain distal nephron epithelium and no ureteric epithelium, this distal nephron segment alone displays significant in vitro plasticity and can adopt a ureteric epithelial tip identity when isolated and cultured in defined conditions. “Induced” ureteric epithelium cultures can be cryopreserved, serially passaged without loss of identity, and transitioned toward a collecting duct fate. Cultures harboring loss-of-function mutations in PKHD1 also recapitulate the cystic phenotype associated with autosomal recessive polycystic kidney disease.